whole tumor segmentation (Danaher Inc)
99
Structured Review
Danaher Inc
whole tumor segmentation
Whole Tumor Segmentation, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 3718 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+segment/LAS+X+Life+Science+Microscope+Software+Platform/pm41707652-616-17-16
Average 99 stars, based on 3718 article reviews
Whole Tumor Segmentation, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 3718 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+segment/LAS+X+Life+Science+Microscope+Software+Platform/pm41707652-616-17-16
Average 99 stars, based on 3718 article reviews
whole tumor segmentation - by Bioz Stars,
2026-10
99/100 stars
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Software:Article Title: Combined swimming with melatonin protects against behavioural deficit in cerebral ischemia-reperfusion injury induced rats associated with modulation of Mst1- MAPK -ERK signalling pathway. Article Snippet: Background: The inconvenience of social and behavioural deficits after cerebral ischaemia reperfusion (I/R) injury is still not well documented.. Aim: We aimed to study the protective effect of preconditioning swimming exercise combined with melatonin against cerebral I/R induced injury.. Methodology: Sixty rats were allocated into 6 groups; groups I and II served as control. Article Title: Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors. Article Snippet: Bio-Optica Cat# 05-9801 Dehyol 95 Bio-Optica Cat# 06-10070Q Avidin/Biotin Blocking Kit Vector Laboratories Cat# SP-2001 M.O.M. (Mouse on Mouse) Blocking Reagent Vector Laboratories Cat# MKB-2213-1 DAPI Sigma-Aldrich Cat# 32670 MOWIOL 4-88 Reagent Merck Cat# 475904-M Collagenase/Dispase Roche Cat# 10269638001 Collagenase P Roche Cat# 11213857001 DNAse I Roche Cat# 11284932001 Liberase Roche Cat# 05401119001 Anti-APC MicroBeads Miltenyi Biotec Cat# 130-090-855 CD45 MicroBeads, mouse Miltenyi Biotec Cat# 130-052-301 MACS LS columns Miltenyi Biotec Cat# 130-042-401 MACS MS columns Miltenyi Biotec Cat# 130-042-201 Critical commercial assays TSA Fluorescein System Akoya Biosciences Cat#NEL701A001KT ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10X Genomics Cat# PN-120237 (Continued on next page) e2 Immunity 55, 606–622.e1–e6, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chromium Single Cell 30 Library Kit 10x Genomics Cat# PN-120234 Chromium Single Cell 30 Gel Bead Kit 10x Genomics Cat# PN-120235 Chromium i7 Multiplex Kit 10x Genomics Cat# PN-120262 Chromium Single Cell A Chip Kit 10x Genomics Cat# PN-120236 Deposited data Single-cell RNaseq data This manuscript NCBI Gene Expression Omnibus (GEO) accession GEO: GSE151623 Experimental models: Organisms/strains Mouse: Hoxb6CreERT Nguyen et al., 2009 Internal colony Mouse: Isl1Cre/+, Sun et al., 2007 Internal colony Mouse: Pax3Cre/+ The Jackson Laboratory Stock No: 005549 Mouse: Ltbrfl/fl Wimmer et al., 2012 Internal colony Mouse: Rosa26-tdTomato The Jackson Laboratory Stock No: 007914 Software and algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific- software/prism FlowJo V10 TreeStar https://www.flowjo.com/ ImageJ V1.5 Imagej N/A Adobe Illustrator CC 2019 Adobe N/A Leica Application Suite X (LAS X) Leica Microsystem N/A Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ CellRanger 10x Genomics https://support.10xgenomics.com/single-cell- gene-expression/software/downloads/latest Other BD FACSCanto II BD Bioscience N/A BD FACSAria III SORP BD Bioscience N/A SP5 or Imaging:Article Title: Combined swimming with melatonin protects against behavioural deficit in cerebral ischemia-reperfusion injury induced rats associated with modulation of Mst1- MAPK -ERK signalling pathway. Article Snippet: Background: The inconvenience of social and behavioural deficits after cerebral ischaemia reperfusion (I/R) injury is still not well documented.. Aim: We aimed to study the protective effect of preconditioning swimming exercise combined with melatonin against cerebral I/R induced injury.. Methodology: Sixty rats were allocated into 6 groups; groups I and II served as control. Expressing:Article Title: The N-terminal GTPase domain of p190RhoGAP proteins is a pseudoGTPase Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS All recombinant proteins were expressed in Rosetta(DE3) cells (Millipore Sigma) grown in Miller’s Luria Broth base (Life Technologies) at 37°C shaking at 200 rpm in a Forma Orbital Shaker (ThermoFisher), with protein expression induced at OD 600 = 0.6 with 0.2 mM isopropyl β-d-thiogalactopyranoside (IPTG) overnight at 16 °C with shaking. .. METHOD DETAILS Expression Constructs The complementary DNA (cDNA) encoding full-length Rattus norvegicus (rat) p190RhoGAP-A (ARHGAP35) protein (NCBI Reference Sequence: {"type":"entrez-protein","attrs":{"text":"NP_001258061.1","term_id":"402794761","term_text":"NP_001258061.1"}} NP_001258061.1 , UniProt: A0A0G2KB46) was used as a PCR-template to amplify the segment encoding residues 1-266 which was inserted into Construct:Article Title: The N-terminal GTPase domain of p190RhoGAP proteins is a pseudoGTPase Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS All recombinant proteins were expressed in Rosetta(DE3) cells (Millipore Sigma) grown in Miller’s Luria Broth base (Life Technologies) at 37°C shaking at 200 rpm in a Forma Orbital Shaker (ThermoFisher), with protein expression induced at OD 600 = 0.6 with 0.2 mM isopropyl β-d-thiogalactopyranoside (IPTG) overnight at 16 °C with shaking. .. METHOD DETAILS Expression Constructs The complementary DNA (cDNA) encoding full-length Rattus norvegicus (rat) p190RhoGAP-A (ARHGAP35) protein (NCBI Reference Sequence: {"type":"entrez-protein","attrs":{"text":"NP_001258061.1","term_id":"402794761","term_text":"NP_001258061.1"}} NP_001258061.1 , UniProt: A0A0G2KB46) was used as a PCR-template to amplify the segment encoding residues 1-266 which was inserted into Sequencing:Article Title: The N-terminal GTPase domain of p190RhoGAP proteins is a pseudoGTPase Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS All recombinant proteins were expressed in Rosetta(DE3) cells (Millipore Sigma) grown in Miller’s Luria Broth base (Life Technologies) at 37°C shaking at 200 rpm in a Forma Orbital Shaker (ThermoFisher), with protein expression induced at OD 600 = 0.6 with 0.2 mM isopropyl β-d-thiogalactopyranoside (IPTG) overnight at 16 °C with shaking. .. METHOD DETAILS Expression Constructs The complementary DNA (cDNA) encoding full-length Rattus norvegicus (rat) p190RhoGAP-A (ARHGAP35) protein (NCBI Reference Sequence: {"type":"entrez-protein","attrs":{"text":"NP_001258061.1","term_id":"402794761","term_text":"NP_001258061.1"}} NP_001258061.1 , UniProt: A0A0G2KB46) was used as a PCR-template to amplify the segment encoding residues 1-266 which was inserted into Polymerase Chain Reaction:Article Title: The N-terminal GTPase domain of p190RhoGAP proteins is a pseudoGTPase Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS All recombinant proteins were expressed in Rosetta(DE3) cells (Millipore Sigma) grown in Miller’s Luria Broth base (Life Technologies) at 37°C shaking at 200 rpm in a Forma Orbital Shaker (ThermoFisher), with protein expression induced at OD 600 = 0.6 with 0.2 mM isopropyl β-d-thiogalactopyranoside (IPTG) overnight at 16 °C with shaking. .. METHOD DETAILS Expression Constructs The complementary DNA (cDNA) encoding full-length Rattus norvegicus (rat) p190RhoGAP-A (ARHGAP35) protein (NCBI Reference Sequence: {"type":"entrez-protein","attrs":{"text":"NP_001258061.1","term_id":"402794761","term_text":"NP_001258061.1"}} NP_001258061.1 , UniProt: A0A0G2KB46) was used as a PCR-template to amplify the segment encoding residues 1-266 which was inserted into Plasmid Preparation:Article Title: The N-terminal GTPase domain of p190RhoGAP proteins is a pseudoGTPase Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS All recombinant proteins were expressed in Rosetta(DE3) cells (Millipore Sigma) grown in Miller’s Luria Broth base (Life Technologies) at 37°C shaking at 200 rpm in a Forma Orbital Shaker (ThermoFisher), with protein expression induced at OD 600 = 0.6 with 0.2 mM isopropyl β-d-thiogalactopyranoside (IPTG) overnight at 16 °C with shaking. .. METHOD DETAILS Expression Constructs The complementary DNA (cDNA) encoding full-length Rattus norvegicus (rat) p190RhoGAP-A (ARHGAP35) protein (NCBI Reference Sequence: {"type":"entrez-protein","attrs":{"text":"NP_001258061.1","term_id":"402794761","term_text":"NP_001258061.1"}} NP_001258061.1 , UniProt: A0A0G2KB46) was used as a PCR-template to amplify the segment encoding residues 1-266 which was inserted into Single Cell:Article Title: Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors. Article Snippet: Bio-Optica Cat# 05-9801 Dehyol 95 Bio-Optica Cat# 06-10070Q Avidin/Biotin Blocking Kit Vector Laboratories Cat# SP-2001 M.O.M. (Mouse on Mouse) Blocking Reagent Vector Laboratories Cat# MKB-2213-1 DAPI Sigma-Aldrich Cat# 32670 MOWIOL 4-88 Reagent Merck Cat# 475904-M Collagenase/Dispase Roche Cat# 10269638001 Collagenase P Roche Cat# 11213857001 DNAse I Roche Cat# 11284932001 Liberase Roche Cat# 05401119001 Anti-APC MicroBeads Miltenyi Biotec Cat# 130-090-855 CD45 MicroBeads, mouse Miltenyi Biotec Cat# 130-052-301 MACS LS columns Miltenyi Biotec Cat# 130-042-401 MACS MS columns Miltenyi Biotec Cat# 130-042-201 Critical commercial assays TSA Fluorescein System Akoya Biosciences Cat#NEL701A001KT ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10X Genomics Cat# PN-120237 (Continued on next page) e2 Immunity 55, 606–622.e1–e6, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chromium Single Cell 30 Library Kit 10x Genomics Cat# PN-120234 Chromium Single Cell 30 Gel Bead Kit 10x Genomics Cat# PN-120235 Chromium i7 Multiplex Kit 10x Genomics Cat# PN-120262 Chromium Single Cell A Chip Kit 10x Genomics Cat# PN-120236 Deposited data Single-cell RNaseq data This manuscript NCBI Gene Expression Omnibus (GEO) accession GEO: GSE151623 Experimental models: Organisms/strains Mouse: Hoxb6CreERT Nguyen et al., 2009 Internal colony Mouse: Isl1Cre/+, Sun et al., 2007 Internal colony Mouse: Pax3Cre/+ The Jackson Laboratory Stock No: 005549 Mouse: Ltbrfl/fl Wimmer et al., 2012 Internal colony Mouse: Rosa26-tdTomato The Jackson Laboratory Stock No: 007914 Software and algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific- software/prism FlowJo V10 TreeStar https://www.flowjo.com/ ImageJ V1.5 Imagej N/A Adobe Illustrator CC 2019 Adobe N/A Leica Application Suite X (LAS X) Leica Microsystem N/A Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ CellRanger 10x Genomics https://support.10xgenomics.com/single-cell- gene-expression/software/downloads/latest Other BD FACSCanto II BD Bioscience N/A BD FACSAria III SORP BD Bioscience N/A SP5 or Multiplex Assay:Article Title: Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors. Article Snippet: Bio-Optica Cat# 05-9801 Dehyol 95 Bio-Optica Cat# 06-10070Q Avidin/Biotin Blocking Kit Vector Laboratories Cat# SP-2001 M.O.M. (Mouse on Mouse) Blocking Reagent Vector Laboratories Cat# MKB-2213-1 DAPI Sigma-Aldrich Cat# 32670 MOWIOL 4-88 Reagent Merck Cat# 475904-M Collagenase/Dispase Roche Cat# 10269638001 Collagenase P Roche Cat# 11213857001 DNAse I Roche Cat# 11284932001 Liberase Roche Cat# 05401119001 Anti-APC MicroBeads Miltenyi Biotec Cat# 130-090-855 CD45 MicroBeads, mouse Miltenyi Biotec Cat# 130-052-301 MACS LS columns Miltenyi Biotec Cat# 130-042-401 MACS MS columns Miltenyi Biotec Cat# 130-042-201 Critical commercial assays TSA Fluorescein System Akoya Biosciences Cat#NEL701A001KT ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10X Genomics Cat# PN-120237 (Continued on next page) e2 Immunity 55, 606–622.e1–e6, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chromium Single Cell 30 Library Kit 10x Genomics Cat# PN-120234 Chromium Single Cell 30 Gel Bead Kit 10x Genomics Cat# PN-120235 Chromium i7 Multiplex Kit 10x Genomics Cat# PN-120262 Chromium Single Cell A Chip Kit 10x Genomics Cat# PN-120236 Deposited data Single-cell RNaseq data This manuscript NCBI Gene Expression Omnibus (GEO) accession GEO: GSE151623 Experimental models: Organisms/strains Mouse: Hoxb6CreERT Nguyen et al., 2009 Internal colony Mouse: Isl1Cre/+, Sun et al., 2007 Internal colony Mouse: Pax3Cre/+ The Jackson Laboratory Stock No: 005549 Mouse: Ltbrfl/fl Wimmer et al., 2012 Internal colony Mouse: Rosa26-tdTomato The Jackson Laboratory Stock No: 007914 Software and algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific- software/prism FlowJo V10 TreeStar https://www.flowjo.com/ ImageJ V1.5 Imagej N/A Adobe Illustrator CC 2019 Adobe N/A Leica Application Suite X (LAS X) Leica Microsystem N/A Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ CellRanger 10x Genomics https://support.10xgenomics.com/single-cell- gene-expression/software/downloads/latest Other BD FACSCanto II BD Bioscience N/A BD FACSAria III SORP BD Bioscience N/A SP5 or RNA sequencing:Article Title: Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors. Article Snippet: Bio-Optica Cat# 05-9801 Dehyol 95 Bio-Optica Cat# 06-10070Q Avidin/Biotin Blocking Kit Vector Laboratories Cat# SP-2001 M.O.M. (Mouse on Mouse) Blocking Reagent Vector Laboratories Cat# MKB-2213-1 DAPI Sigma-Aldrich Cat# 32670 MOWIOL 4-88 Reagent Merck Cat# 475904-M Collagenase/Dispase Roche Cat# 10269638001 Collagenase P Roche Cat# 11213857001 DNAse I Roche Cat# 11284932001 Liberase Roche Cat# 05401119001 Anti-APC MicroBeads Miltenyi Biotec Cat# 130-090-855 CD45 MicroBeads, mouse Miltenyi Biotec Cat# 130-052-301 MACS LS columns Miltenyi Biotec Cat# 130-042-401 MACS MS columns Miltenyi Biotec Cat# 130-042-201 Critical commercial assays TSA Fluorescein System Akoya Biosciences Cat#NEL701A001KT ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10X Genomics Cat# PN-120237 (Continued on next page) e2 Immunity 55, 606–622.e1–e6, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chromium Single Cell 30 Library Kit 10x Genomics Cat# PN-120234 Chromium Single Cell 30 Gel Bead Kit 10x Genomics Cat# PN-120235 Chromium i7 Multiplex Kit 10x Genomics Cat# PN-120262 Chromium Single Cell A Chip Kit 10x Genomics Cat# PN-120236 Deposited data Single-cell RNaseq data This manuscript NCBI Gene Expression Omnibus (GEO) accession GEO: GSE151623 Experimental models: Organisms/strains Mouse: Hoxb6CreERT Nguyen et al., 2009 Internal colony Mouse: Isl1Cre/+, Sun et al., 2007 Internal colony Mouse: Pax3Cre/+ The Jackson Laboratory Stock No: 005549 Mouse: Ltbrfl/fl Wimmer et al., 2012 Internal colony Mouse: Rosa26-tdTomato The Jackson Laboratory Stock No: 007914 Software and algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific- software/prism FlowJo V10 TreeStar https://www.flowjo.com/ ImageJ V1.5 Imagej N/A Adobe Illustrator CC 2019 Adobe N/A Leica Application Suite X (LAS X) Leica Microsystem N/A Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ CellRanger 10x Genomics https://support.10xgenomics.com/single-cell- gene-expression/software/downloads/latest Other BD FACSCanto II BD Bioscience N/A BD FACSAria III SORP BD Bioscience N/A SP5 or Gene Expression:Article Title: Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors. Article Snippet: Bio-Optica Cat# 05-9801 Dehyol 95 Bio-Optica Cat# 06-10070Q Avidin/Biotin Blocking Kit Vector Laboratories Cat# SP-2001 M.O.M. (Mouse on Mouse) Blocking Reagent Vector Laboratories Cat# MKB-2213-1 DAPI Sigma-Aldrich Cat# 32670 MOWIOL 4-88 Reagent Merck Cat# 475904-M Collagenase/Dispase Roche Cat# 10269638001 Collagenase P Roche Cat# 11213857001 DNAse I Roche Cat# 11284932001 Liberase Roche Cat# 05401119001 Anti-APC MicroBeads Miltenyi Biotec Cat# 130-090-855 CD45 MicroBeads, mouse Miltenyi Biotec Cat# 130-052-301 MACS LS columns Miltenyi Biotec Cat# 130-042-401 MACS MS columns Miltenyi Biotec Cat# 130-042-201 Critical commercial assays TSA Fluorescein System Akoya Biosciences Cat#NEL701A001KT ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10X Genomics Cat# PN-120237 (Continued on next page) e2 Immunity 55, 606–622.e1–e6, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chromium Single Cell 30 Library Kit 10x Genomics Cat# PN-120234 Chromium Single Cell 30 Gel Bead Kit 10x Genomics Cat# PN-120235 Chromium i7 Multiplex Kit 10x Genomics Cat# PN-120262 Chromium Single Cell A Chip Kit 10x Genomics Cat# PN-120236 Deposited data Single-cell RNaseq data This manuscript NCBI Gene Expression Omnibus (GEO) accession GEO: GSE151623 Experimental models: Organisms/strains Mouse: Hoxb6CreERT Nguyen et al., 2009 Internal colony Mouse: Isl1Cre/+, Sun et al., 2007 Internal colony Mouse: Pax3Cre/+ The Jackson Laboratory Stock No: 005549 Mouse: Ltbrfl/fl Wimmer et al., 2012 Internal colony Mouse: Rosa26-tdTomato The Jackson Laboratory Stock No: 007914 Software and algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific- software/prism FlowJo V10 TreeStar https://www.flowjo.com/ ImageJ V1.5 Imagej N/A Adobe Illustrator CC 2019 Adobe N/A Leica Application Suite X (LAS X) Leica Microsystem N/A Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ CellRanger 10x Genomics https://support.10xgenomics.com/single-cell- gene-expression/software/downloads/latest Other BD FACSCanto II BD Bioscience N/A BD FACSAria III SORP BD Bioscience N/A SP5 or Microscopy:Article Title: Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors. Article Snippet: Bio-Optica Cat# 05-9801 Dehyol 95 Bio-Optica Cat# 06-10070Q Avidin/Biotin Blocking Kit Vector Laboratories Cat# SP-2001 M.O.M. (Mouse on Mouse) Blocking Reagent Vector Laboratories Cat# MKB-2213-1 DAPI Sigma-Aldrich Cat# 32670 MOWIOL 4-88 Reagent Merck Cat# 475904-M Collagenase/Dispase Roche Cat# 10269638001 Collagenase P Roche Cat# 11213857001 DNAse I Roche Cat# 11284932001 Liberase Roche Cat# 05401119001 Anti-APC MicroBeads Miltenyi Biotec Cat# 130-090-855 CD45 MicroBeads, mouse Miltenyi Biotec Cat# 130-052-301 MACS LS columns Miltenyi Biotec Cat# 130-042-401 MACS MS columns Miltenyi Biotec Cat# 130-042-201 Critical commercial assays TSA Fluorescein System Akoya Biosciences Cat#NEL701A001KT ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10X Genomics Cat# PN-120237 (Continued on next page) e2 Immunity 55, 606–622.e1–e6, April 12, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chromium Single Cell 30 Library Kit 10x Genomics Cat# PN-120234 Chromium Single Cell 30 Gel Bead Kit 10x Genomics Cat# PN-120235 Chromium i7 Multiplex Kit 10x Genomics Cat# PN-120262 Chromium Single Cell A Chip Kit 10x Genomics Cat# PN-120236 Deposited data Single-cell RNaseq data This manuscript NCBI Gene Expression Omnibus (GEO) accession GEO: GSE151623 Experimental models: Organisms/strains Mouse: Hoxb6CreERT Nguyen et al., 2009 Internal colony Mouse: Isl1Cre/+, Sun et al., 2007 Internal colony Mouse: Pax3Cre/+ The Jackson Laboratory Stock No: 005549 Mouse: Ltbrfl/fl Wimmer et al., 2012 Internal colony Mouse: Rosa26-tdTomato The Jackson Laboratory Stock No: 007914 Software and algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific- software/prism FlowJo V10 TreeStar https://www.flowjo.com/ ImageJ V1.5 Imagej N/A Adobe Illustrator CC 2019 Adobe N/A Leica Application Suite X (LAS X) Leica Microsystem N/A Seurat (v3.2.2) Stuart et al., 2019 https://satijalab.org/seurat/ CellRanger 10x Genomics https://support.10xgenomics.com/single-cell- gene-expression/software/downloads/latest Other BD FACSCanto II BD Bioscience N/A BD FACSAria III SORP BD Bioscience N/A SP5 or |